MUT'n'CUT (beta testing)
Identifies Unique Restriction Enzyme Sites Generated by Single Nucleotide Mutation.
How to use this program
How To Use This Program
The purpose of MUT'n'CUT is to define the location of nucleotide changes which would
generate unique restriction enzyme recognition sites in a
user-defined sequence. Furthermore, the program determines the effects the nucleotide
mutation would have if it were to occur within an open
reading frame (ORF). This permits the researcher to eliminate or minimize deleterious
effects on the gene product, and hence in the organism.
The entire sequence is first analyzed to determine which enzyme recognitions sites
are present. These enzymes are excluded from further analysis. The reading frame is
derived from the value entered in the field ORF start. FROM and TO define the region
searched for mutation sites.
At this time MUT'n'CUT does not consider mutations resulting from changes to more than one nucleotide.
This program is presented here for beta testing
last modified 8/11/00
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